Sequencing gel solution premixes
e.power at umds.ac.uk
Wed Jul 12 10:40:40 EST 1995
mwg at mail.nerc-oxford.ac.uk (Mike Gaunt) wrote:
>Dear Sequencers and Associates:
>I was wondering if a standard urea, acrylamide, TBE, water and TEMED solution
>was stable during storage. I ask with regards to the companies (e.g. National
>Diagnostics) selling sequencing gel solutions which are ready to set after
>the addtion of APS.
We have used some of these pre-mixed solutions in our lab. They seem to
be stable but we consistently found that the resolution and quality of
our gels was not as good as our 'home-made' freshly made stuff. These
days we only use pre-mixed acrylamide solutions and make up and add the
other components as required. So far, so good!
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