Sequencing gel solution premixes

[Eddie Power]

mwg at mail.nerc-oxford.ac.uk (Mike Gaunt) wrote:
>
>Dear Sequencers and Associates:
>
>I was wondering if a standard urea, acrylamide, TBE, water and TEMED solution 
>was stable during storage. I ask with regards to the companies  (e.g. National 
>Diagnostics) selling sequencing gel solutions which are ready to set after 
>the addtion of APS. 
>
>Cheers 
>
>Mike
> 

We have used some of these pre-mixed solutions in our lab. They seem to 
be stable but we consistently found that the resolution and quality of 
our gels was not as good as our 'home-made' freshly made stuff. These 
days we only use pre-mixed acrylamide solutions and make up and add the 
other components as required. So far, so good!

Good luck!


Eddie