DNA purification from agarose gels

L. Hays lghays at U.WASHINGTON.EDU
Thu Jul 13 23:28:59 EST 1995


On 13 Jul 1995, Tim wrote:

> In article <bio.tamu.edu-2906951005260001 at ricelab-centris650.tamu.edu>
> bio.tamu.edu writes:
> >I am interested in what other people think about Qiagens new Qiaquick
> >columns compared to Promega's wizard PCR preps.  Has anybody done a direct
> >comparison of these two kits for purification of DNA from gels?
> 
> We have not used the Qiagen kit but have had poor yields with the
> Promega kit.
> When we have attempted to purify plasmids (>3kb) with the Promega kit
> we have consistently
> recovered a 1.5kb.  All of our reagents have been filtered, thus it is
> unlikely 
> that this a contaminating band.  To date we have 0% yield of the
> desired DNA.  
> and
> I would be interested in hearing from anyone else who has had similar
> problems.   
> 
> t.kuwada at neuron.uchc.edu
> 
> 

I have also poor results from Promega's Wizard kits.  They are 
inconsistent.  Sometimes they do produce goo yield.  I have also used 
Qiagen's kit.  It works well.

Lara G. Hays lghays at u.washington.edu
       .***.   .***.           .***.   .***.           .***.   .***.
Department of Molecular Biotechnology* | | | *       * | | | * | | | *    
University of Washington           *   * | | | *   * | | | *   * | | | * 
Box 352145, Health Sciences, K307*       * | | | * | | | *       * | | | *
Seattle, WA  98195 '***'   '***'           '***'   '***'           '***'





More information about the Methods mailing list