DNA purification from agarose gels
lghays at U.WASHINGTON.EDU
Thu Jul 13 23:28:59 EST 1995
On 13 Jul 1995, Tim wrote:
> In article <bio.tamu.edu-2906951005260001 at ricelab-centris650.tamu.edu>
> bio.tamu.edu writes:
> >I am interested in what other people think about Qiagens new Qiaquick
> >columns compared to Promega's wizard PCR preps. Has anybody done a direct
> >comparison of these two kits for purification of DNA from gels?
> We have not used the Qiagen kit but have had poor yields with the
> Promega kit.
> When we have attempted to purify plasmids (>3kb) with the Promega kit
> we have consistently
> recovered a 1.5kb. All of our reagents have been filtered, thus it is
> that this a contaminating band. To date we have 0% yield of the
> desired DNA.
> I would be interested in hearing from anyone else who has had similar
> t.kuwada at neuron.uchc.edu
I have also poor results from Promega's Wizard kits. They are
inconsistent. Sometimes they do produce goo yield. I have also used
Qiagen's kit. It works well.
Lara G. Hays lghays at u.washington.edu
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