Ligation in 5 min!

Tim tkuwada at
Thu Jul 13 15:58:42 EST 1995

oshiro_G at (Guy Oshiro) wrote:
>Why do you need a kit to do a quick ligation.  The ligation reaction
>proceeds very quickly at R.T. without any special tricks involved.  The
>ligation rxn is usually 90% complete within 15'.

Is your DNA purified or in agarose?.  Have you tried a 16C rxn temp and
if so how 
does it compare?

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