oshiro_G at defiance.hsc.colorado.edu (Guy Oshiro) wrote: >Why do you need a kit to do a quick ligation. The ligation reaction >proceeds very quickly at R.T. without any special tricks involved. The >ligation rxn is usually 90% complete within 15'. Is your DNA purified or in agarose?. Have you tried a 16C rxn temp and if so how does it compare?