hekp with sigmacoat and sequencing gels

[Colin Rasmussen]

I guess I'm a little confused by all this talk of unreliability.  I wash 
my plates in water, wipe them dry then spray on some 95% ethanol and 
wipe that dry.  I then sprinkle on a little Sigmacote and then spray on 
a little water and wipe that dry. I do both plates and never have a 
problem with bubbles or getting the gel on the plate I want.

Colin