Lysing yeast spheroplasts

[Manuel .G CLAROS]

In Article <3u1cjb$5et at seymour.sfu.ca>, wasylnka at sfu.ca (Julie Wasylnka) wrote:

>I am trying to lyse yeast spheroplasts in order to make yeast DNA.  I 
>have a protocol which used DTT. I was wondering if I could substitute 2-ME 
>for DTT and what concentration I should use? (The protocol suggests 1.8 
>mM final DTT).

I use SDS 1% final and works fine.

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