Sequencing gel solution premixes
R. P. Grant +44 1 865 221018
rpgrant at molbiol.ox.ac.uk
Thu Jul 13 12:15:48 EST 1995
In article <Pine.SGI.3.91.950712153522.29496A-100000 at lonestar.jpl.utsa.edu>, "Billy B. Long" <blong at lonestar.jpl.utsa.edu> writes:
> Hi, Richard.
And various people.
I DID NOT WRITE THE ORIGINAL POST.
Thank you for your kind concern though ;)
We actually use Nat Diagnostic (I think off hand) reagents now.
Someone else>> > I was wondering if a standard urea, acrylamide, TBE, water and TEMED solution
>> > was stable during storage. I ask with regards to the companies (e.g. National
>> > Diagnostics) selling sequencing gel solutions which are ready to set after
>> > the addtion of APS.
ME>> Dunno about the TMED, but we used to make up a 10/9 stock of Urea/Ac in h2o,
>> deionize and filter. Kept for months at 4C (in the dark).
>> Then we added 10XTBE (filtered, if necessary) and the AMPS + TEMED.
>> I'd worry about the TBE ppting if stored cold.
Thank you every one who sent me email. But I didn't want to know!
Richard P. Grant MA DPhil rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
FAX +44 1 865 69141 TEL +44 1 865 221018
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