In vitro transcription, Ambion's secret?

[Paul E. Ulanch]

>Promega makes a similar high yield transcription kit which they
>described in a recent issue of Promega Notes.  The article was very
>complete including buffer recipes and final concentrations of all
>the other components.  When I copied the protocol, it worked
>fabulously.  I'm sorry I don't have the issue number right now
>because I'm at home on holidays.  If you can't find it, e-mail me
>next week when I'm back in the lab and I'll send you the details.
>Jill

Are you sure that this is a 'Mega' kit or just the recipes for normal in vitro 
transcription reactions for those polymerases (T3,T7,SP6)?  Epicentre Tech. 
also makes a kit similar to Ambion's called Ampliscribe and it works well 
also, but I don't know the secrets.  I would like to know though, please reply 
to me if you figure it out!


Paul "Goose" Ulanch

Dept. Biochem/Biophys
Texas A&M University
College Station, TX   77843-2128
(409) 847-9379
PULANCH at bioch.tamu.edu