Southern Hybridization Buffers?
aquilla at salus.med.uvm.edu
Thu Jul 13 07:13:45 EST 1995
In Article <stewart-1207950928370001 at mac51.ciit.org>, stewart at ciit.org
(Wendy Stewart) wrote:
>In article <3tpt6d$chq at agate.berkeley.edu>, Tjian lab
><insomnia at mendel.berkeley.edu> wrote:
>> Here's my recipe for Church's buffer. IMHO, it's the best general
>> purpose hyb buffer I've ever tried. Low background, easy to use, nice
>> 0.5M Na phosphate, pH 7.2 (0.5M in Na, so 134g/l of Na2HPO4+7H2O,
>> pH'ed with 4ml 85% phosphoric acid)
>> 7% SDS
>> 0.5mM EDTA
>I also add 1%BSA to the above recipe.
>These are my opinions only and do not reflect that of my company
Try 10% SDS. That's all; it works!
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