Southern Hybridization Buffers?

Tracy Aquilla aquilla at salus.med.uvm.edu
Thu Jul 13 07:13:45 EST 1995


In Article <stewart-1207950928370001 at mac51.ciit.org>, stewart at ciit.org
(Wendy Stewart) wrote:
>In article <3tpt6d$chq at agate.berkeley.edu>, Tjian lab
><insomnia at mendel.berkeley.edu> wrote:
>
>> Here's my recipe for Church's buffer.  IMHO, it's the best general 
>> purpose hyb buffer I've ever tried.  Low background, easy to use, nice 
>> signals.
>> 
>> 0.5M Na phosphate, pH 7.2 (0.5M in Na, so 134g/l of Na2HPO4+7H2O,
>>                             pH'ed with 4ml 85% phosphoric acid)
>> 7% SDS
>> 0.5mM EDTA
>> 
>I also add 1%BSA to the above recipe.
>
>-- 
>These are my opinions only and do not reflect that of my company

Try 10% SDS. That's all; it works!
    tracy



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