GST fusion prots truncating at 27 kDa?

Lisa Estey lisa_estey at glaxo.com
Thu Jul 13 08:31:22 EST 1995


Jeff- 
	You may want to try induction at lower temperatures (our lab uses 20°C) 
in order to increase the stability of your fusions.  Another trick you 
may want to try is to use BL21 as your host strain- these cells carry 
mutations in lon and ompT proteases.

Hope this helps!

-Lisa Estey  
 Just another frustrated postdoc





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