Mammalian Electroporation?!?

Dr Jonathan Backer backer at aecom.yu.edu
Thu Jul 13 08:25:37 EST 1995


In article <3u31k6$218 at sifon.cc.mcgill.ca>, Graham Dellaire <popa0206 at PO-Box.McGill.CA> says:
>
>HAs anyone out there obtained an electroporation frequency
>better than 10-5 transformants/no# cells transformed???
>
>
Have a look at a JBC paper by Yamauchi and Pessin (late 1994), where they get an extremely high rate of transfection
in CHO cells. It requires killing most of the cells, but the rest are nearly all blue (with b-gal).

Jon Backer>



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