harvesting DNA from a lambda gt11 clone

patricia benziman at vms.huji.ac.il
Thu Jul 13 10:29:50 EST 1995


I am trying to obtain a clean lambda gt11 DNA prep. for sequencing without any success. 
I used Quiagen kit but the yield was low and the ratio 260/280 was always
less than 1.7. I also tried the Maniatis method using SDS lysis, with no
great success. Does anyone have a suggestion? Thanks. Patricia.



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