GFP expression in Fibroblasts and K46 B-cells

Ulrich Thoenes thoenes at itc-biotech.com
Tue Jul 11 09:25:42 EST 1995


Dear Bionetters,

Is there anybody who has experience in expressing the Green
Fluorescent Protein using the pGFP-N1 vector in K46 B-cells from mouse
as well as in fibroblasts?
The fibroblasts were primary fibroblasts imortalized with SV40. From
this a cell line was built to be used for the expression of GFP.

Thanks, U.Thoenes
 




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