Sma I / PEG

[R. P. Grant +44 1 865 221018]

In article <3u0pv5$eja at rover.ucs.ualberta.ca>, Colin Rasmussen <colin_rasmussen at darwin.biochem.ualberta.ca> writes:
> We always use the BRL ligation buffer which contains PEG-8000 and do the 
> SmaI digests at 25°C. We usually have very few problems.
> 


Never used PEG in my life.  One time, we did a direct comparison and found 
that PEG decreased number of recombinant colonies to zero.

Go figure, huh?

Richard
-- 
Richard P. Grant MA DPhil      rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
FAX +44 1 865 69141            TEL +44 1 865 221018
http://sable.ox.ac.uk/~lady0266

The fecal material has impacted on the air circulating device.