Sma I / PEG
R. P. Grant +44 1 865 221018
rpgrant at molbiol.ox.ac.uk
Thu Jul 13 04:29:28 EST 1995
In article <3u0pv5$eja at rover.ucs.ualberta.ca>, Colin Rasmussen <colin_rasmussen at darwin.biochem.ualberta.ca> writes:
> We always use the BRL ligation buffer which contains PEG-8000 and do the
> SmaI digests at 25°C. We usually have very few problems.
Never used PEG in my life. One time, we did a direct comparison and found
that PEG decreased number of recombinant colonies to zero.
Go figure, huh?
Richard P. Grant MA DPhil rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
FAX +44 1 865 69141 TEL +44 1 865 221018
The fecal material has impacted on the air circulating device.
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