RNA gels - staining and denaturing
ddh at unlinfo.unl.edu
Fri Jul 14 15:04:14 EST 1995
Amy Marion (amarion at moose.uvm.edu) wrote:
: Dear netters;
: I need some advice on RNA gels. I have made 3 attempts to run
: total RNA through glyoxal/DMSO gels and one attempt with a formamide gel.
: In all cases I have been unable to see the RNA after ethidium bromide
: staining. Even the RNA markers do not show up.
: Should RNA be stained with ethidium bromide at all? (These gels will be
: used for Northerns.)
: What is the proper procedure for staining RNA gels (either glyoxal or
: formamide) with ethidium bromide?
: I have run the RNA through 0.1% SDS, 1.2% agarose gels. After
: staining with EtBr all the RNA (including the RNA markers) are visible.
: Will the 0.1% SDS denature the RNA? If yes, can the RNA from this gel be
: transfered to nylon for a Northern?
: Thanks for your help.
: Amy Marion
: marion at smtplink.ipfw.indiana.edu
Just use 1 ul of 0.4 ug/ul for 50 ul RNA solution (containing 10 ug
RNA). This should difinitely stain very well two rRNA bands and your
Dinakar Bhattramakki/Ph.:(402) 472-6243/email:ddh at unlinfo.unl.edu
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