Designing PCR primers-How to check them?
Peter---bkxo at musicb.mcgill.ca
Peter---bkxo at musicb.mcgill.ca
Fri Jul 14 14:13:52 EST 1995
I have designed a set of primers for amplification of a 300 bp ds DNA
fragment from human genomic DNA.
I would like to check these primers for any complementarity they may
have to repetitive sequences found in the human genome. I am
concerned that the presence of such complemantarity would result in
non-specific amplification.
Is there a database I can access to perform this search? If yes, how?
I am interested to know how other people go about checking primers
which they have designed before actually ordering them.
You can reach me at bkxo at musicb.mcgill.ca or post a note here.
Thanx.
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