yeast/mammalian expression plasmids

Scott Vande Pol sbv at
Fri Jul 14 11:41:50 EST 1995

Has anyone made a cDNA expression plasmid that expresses well in both
saccharomyces cereciasiae and mammalian cells? Many promoters work in both
cell types, but what about RNA processing signals like splice/polyA.  It
might be possible to incorporate all the signals that both organisms need. 
Does anyone know if such a plasmid has already been constructed? 

Scott Vande Pol
Case Western Reserve University
Department of Pathology
Cleveland Oh. 44106
sbv at

More information about the Methods mailing list