PCR on fresh plant tissue?

[Paul St. Amand]

In article <3u4p2n$o64 at news.iastate.edu>, annieliu at iastate.edu (Tsu-Hwie
Liu) wrote:

PSt> Does anybody know how to do PCR on fresh plant tissue? I have some small
PSt> putative transgenic plants with GUS gene. They are too small for me to do
PSt> DNA extraction and sourthern hybridization. Can I use a small portion of
PSt> plant to amplify GUS gene by PCR? 
PSt> 
PSt> Thanks for any information about it.
PSt> 
PSt> -- 
PSt> Tsu-Hwie Annie Liu
PSt> annieliu at iastate.edu

Tsu-Hwie,

We use the NaOH extraction method (NAR 1993 21:4153-4154) for PCR or RAPD
on single leaves or even half of a cotyledon. Works great after you find
the optimum dilution for your species. It is also very cheap, easy, and
fast. We have stored extracted samples frozen (-80C) for up to 1 year so
far, and they are still good for PCR.

Good luck!

Paul C. St. Amand    (PST at KSU.KSU.Edu)
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