Designing PCR primers-How to check them?
popa0206 at PO-Box.McGill.CA
Fri Jul 14 16:09:41 EST 1995
> Peter --- bkxo at musicb.mcgill.ca writes:
> I have designed a set of primers for amplification of a 300 bp ds DNA
> fragment from human genomic DNA.
> I am interested to know how other people go about checking primers
> which they have designed before actually ordering them.
> You can reach me at bkxo at musicb.mcgill.ca or post a note here.
Unfortunately the only way you will really find out is by using them first and seeing how many bands
What is it for? ... sometimes more than one band is good (as in VNTR analysis) if you are typing
DNA or looking for markers.
Graham Dellaire Snail Mail:
Red Cross, Research
McGill University Montreal Blood Services
Faculty of Medicine 3131 Sherbrooke St. East
Div. of Experimental Medicine Montreal, QC, Canada
E-mail: popa0206 at po-box.mcgill.ca H1W 1B2
B2XE at musicb.mcgill.ca
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