DNA recovery from gels
geneh at USA.NET
Sat Jul 15 12:28:55 EST 1995
In article <bicfo.6.3002D512 at flinders.edu.au|
bicfo at flinders.edu.au writes:
>Gidday, fellow netters!
>I'm having truckloads of trouble trying to consistently recover DNA
>(0.7-1.8kb) from low-melting point agarose gels. I've tried agarase digestion,
>phenol extraction, Promega WIZARD PCR columns and 'squeeze-freeze'
>techniques - all with limited success (none at all with the agarase!). Does
>anybody have a foolproof method, or can anyone direct me to somewhere on the
>net where different techniques might be discussed?
We've been using silica particles for purifying DNA from LMT agarose with
recoveries near 50-70% depending upon who's doing it. (See NAR 21:1044, 93)
This is similar to the Merlin method which can be found in the archives.
In the same vein can anyone describe their experiences with the 'gene
capsules' from Geno Technology?
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