RNA gels - staining and denaturing
dkim at nmsu.edu
Sat Jul 15 13:49:05 EST 1995
Re: RNA gels and ethidium bromide:
I have found glyoxal/DMSO gels also to work well. The ethidium bromide
may not stain RNA as well as DNA, because of the single stranded
structure. Appropriate hydrophobic regions (base stacks) may not appear
as often in RNA because of this, so there may not be as much
fluorescence. How much RNA are you running on a lane?
An alternative would be to stain with Acridine Orange, which can
distinguish between ds and ss nucleic acids, giving different
fluorescence for each.
More information about the Methods