DNA purification of lambda gt11 clones

patricia ohana benziman at vms.huji.ac.il
Sun Jul 16 03:00:23 EST 1995


I am trying to purify DNA from a single lambda gt11 clone in preparation for
direct sequencing. Using the Qiagen kit my yields have been low and the
OD 260/280 ratio is less than 1.7. I have been using plate lysates as starting
material, using about 10 plates containing confluent lysis.
Does anyone have any suggestions?



More information about the Methods mailing list