DNA recovery from gels

giorgio spagnol at galactica.it
Sun Jul 16 07:57:11 EST 1995


bicfo at flinders.edu.au Wrote:
> I'm having truckloads of trouble trying to consistently recover DNA 
> (0.7-1.8kb) from low-melting point agarose gels. I've tried agarase
digestion, 
> phenol extraction, Promega  WIZARD PCR columns  and 'squeeze-freeze' 
> techniques - all with limited success (none at all with the agarase!).
Does 
> anybody have a foolproof method, or can anyone direct me to somewhere
on the 
> net where different techniques might be discussed?
> Thanks, Colleen.

You may try Geneclean, from Biogen, I believe, or any other glass
powder-based extraction method. Is absolutely hassle-free.



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