DNA extraction from Tobacco/Petunia

anon at lamar.colostate.edu anon at lamar.colostate.edu
Mon Jul 17 16:45:34 EST 1995


I need a reliable protocol to extract and isolate genomic DNA from
tobacco.  We are a maize lab and we are concerned about interference from
secondary metabolites.  Are there any particular "tricks" I need to be
aware of?

I also need to know what restriction enzyme produces good fragment lengths
for Southerns.  Can anyone help?

We need to do Petunia DNA as well.  Info on this would also be appreciated.

Thanks.



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