Home-Made TA cloning

Makoto Kamei m-kamei at schooner.waite.adelaide.edu.au
Mon Jul 17 19:16:48 EST 1995


>Surely that should be ddTTP. If you use just dTTP then terminal transferase
>will add umpteen 'T's.

It is dTTP. I've done this and it worked quite well. My superviser was
boasting that this works better than pGEM-T cloning system from Promega.
She was saying that bluescript works better than pGEM for colour
selection.



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