Reusing "disposable" electroporation cuvettes

Dima Klenchin klenchin at macc.wisc.edu
Mon Jul 17 19:13:36 EST 1995


>We recently acquired a BTX E. coli TransPorator which came 
with 50
>disposable cuvettes.
>We're wondering how many times we can reuse these 
"disposable" cuvettes
>before we
>discard them.  Are there any signs to indicate when they 
should be thrown out?
>

Clean as any labware, soak in 70% EtOH, rince with sterile 
water. Generally it's fine to reuse them 2-3 times, but be 
aware that the probability of arcing encreases 
significantly with every use. 

-dima



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