Direct PCR from E. coli colonies

Rick Tearle bresatec at camtech.com.au
Mon Jul 17 07:35:05 EST 1995


pbv at rs.uovs.ac.za (B VISSER * x2818 Plantkunde *) wrote:
 stuff deleted
 I want to amplify the inserts
>directly from E. coli colonies.  Can the same protocol used for the phage DNA be used directly for amplification of DNA from these colonies?  If not, could you suggest a publication where I could find a suitable protocol?

Just pick your colony, streak on a sector plate and inoculate an Eppendorf containing your PCR mix. A 3' 94 deg heating step before starting cycling is enough to denature the DNA.
Rick





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