3kB PCR Cycling Conditions?//

Marc A. Borbely burbly at minerva.cis.yale.edu
Mon Jul 17 01:05:19 EST 1995


Hello. I'm trying to amplify a 3kB fragment from mouse genomic DNA. I've 
been using Taq polymerase with a 3 minute extension time, and 20 seconds 
annealing, and 20 seconds denaturing. I'm getting no product. Does anyone 
have better cycling conditions I should try? 
Thanks

- Marc Borbely



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