Sma I religation problem

Antonio Rodriguez Franco bb1rofra at seneca.uco.es
Mon Jul 17 12:20:53 EST 1995


In article <3tv50s$p5g at dingo.cc.uq.oz.au> mikep at biosci.uq.oz.au (Mike Poidinger) writes:
>From: mikep at biosci.uq.oz.au (Mike Poidinger)
>Subject: Sma I religation problem
>Date: Tue, 11 Jul 1995 23:27:02 GMT

>Hello everyone,

>I have been having major troubles with religating Sma I cut pGEM.  The vector
>has been cut, and the linear plasmid gel purified using Wizard clean up system,
>but try as I might it wont religate to itself.  The same routine performed on
>the same plasmid with Eco RI works just fine.

>Any help anyone has would be greatly appreciated.

>advTHANKSance,
>Mike

>------------------------------------------------------------------------------
>Dr Mike Poidinger      Now don't be lazy, 
>Microbiology, UQ       with the pleasure of sin  (Nitzer Ebb)
>Australia              
>mikep at biosci.uq.oz.au  
>-----------------------------------------------------------------------------

I understand your problem could be treated in a different way.




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