DNA & Microtiter Plates

Roger Wiegand rcwieg at ccmail.monsanto.com
Mon Jul 17 15:13:28 EST 1995


In article <DBpt1u.57y at crash.cts.com>, allang at cts.com wrote:

>I am looking for a method to block polystyrene microtiter plates so that
I can perform a DNA 
>hybridization in them.  If any one has a blocking method that works I
would greatly appreciate any 
>suggestions or help, please email me directly (email = alllang at cts.com)  
Thanks in advance.
>


I found that standard Southern reagents (eg Denhardt's, sonicated calf
thymus DNA, and/or tRNA) for about 15 min at 37 deg work just fine and
they're already in the fridge.

-- 

Thanks,
Roger

mailto::rcwieg at ccmail.monsanto.com



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