Coating LB plates with tet after pouring?

Nathan O. Siemers nathan at
Mon Jul 17 14:53:45 EST 1995

Hello, World.

I only occasionally use tet or tet-kan plates, mainly for maintaining
pili in E. coli.  Problem is, the tetracycline goes bad quickly.  Can
you spread a tet solution over the plate, allow it to dry (and
diffuse), then spread or streak your bacteria?  If so, what
concentration of tet is reasonable to use?



Nathan O. Siemers, Ph.D.	
Bristol-Myers Squibb Pharmaceutical Research Institute
3005 First Avenue, Seattle, WA 98121
(206) 727-3741  	siemers at
"You want fries with that?"

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