Coating LB plates with tet after pouring?

[Nathan O. Siemers]

Hello, World.

I only occasionally use tet or tet-kan plates, mainly for maintaining
pili in E. coli.  Problem is, the tetracycline goes bad quickly.  Can
you spread a tet solution over the plate, allow it to dry (and
diffuse), then spread or streak your bacteria?  If so, what
concentration of tet is reasonable to use?

Thanks,

nathan

--
Nathan O. Siemers, Ph.D.	
Bristol-Myers Squibb Pharmaceutical Research Institute
3005 First Avenue, Seattle, WA 98121
(206) 727-3741  	siemers at bms.com
"You want fries with that?"