Direct PCR from E. coli colonies

[David Karaolis]

I just take bacterial colonies (from a pure growth), inoculate 200ul of 
sterile distilled water, boil for 5min, spin for 5min and use 5ul of 
supernatant as template per 100ul PCR reaction. Works great.
J. Bacteriol. 1995. 177:3191-3198

David K. R. Karaolis

Center for Vaccine Development 
University of Maryland School of Medicine
Baltimore MD  21201  USA

phone:  410-706 2493
fax:  410-706 6205
email:  karaolis at umabnet.ab.umd.edu