PCR GC-rich genomic DNA

Graham Dellaire popa0206 at PO-Box.McGill.CA
Tue Jul 18 22:15:26 EST 1995

>   Steven Goldberg <goldberg at bms.com> writes:
>  Have you tried hot-start?  This procedure has at times (not always, 
>  unfortunately) dramatically improved the efficiency and specificity of 
>  our PCR reactions from high G + C genomes.
>  Good luck.
>  Steve
Look in this months Biotechniques there is an article on GC rich PCR and has a protocol


Graham Dellaire			    Snail Mail:
                                    Red Cross, Research		
McGill University                   Montreal Blood Services	  	
Faculty of Medicine                 3131 Sherbrooke St. East         
Div. of Experimental Medicine       Montreal, QC, Canada           
E-mail: popa0206 at po-box.mcgill.ca   H1W 1B2			   
B2XE at musicb.mcgill.ca							   
WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html	
Fax: (514) 525 0881							   	
Voice: (514) 527 1501 ext 175 						  

More information about the Methods mailing list