help needed about western blots

Martin Hughes mhughes at mcmaster.ca
Tue Jul 18 12:45:47 EST 1995


In article <gslab1.25.0009884C at unixg.ubc.ca>, gslab1 at unixg.ubc.ca (Grad Student Lab 1) says:
>
>Hi, netters. I have a question about western blots. The signal I got was very 
>very faint after I developed my blots with NBT/BCIP for 15 min (protein 
>loading amount was 80 µg/lane).


Soak the blots in boilin hot 0.1% sds the start over 



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