silver staining

Pierre-Alain Menoud menoud at cmu.unige.ch
Tue Jul 18 02:07:57 EST 1995


In article <3udp2d$t1e at galactica.galactica.it>,
   giorgio <spagnol at galactica.it> wrote:
>to silver-stain a polyacrilamide gel I usually waste 3 hours.
>Is there any method or kit available to shorten this time?

Hi,
Have a look in "Current protocols in molecular biology" page 10.6.5. I always 
use this protocols which does not take longer than 30 min. to get an excellent 
staining of either protein or nucleic acid. It is based on Bloom et al. 1987. 
Electrophoresis 8:93-99.

Hope this will help

Pierre-Alain Menoud

Laboratory of Oncology
University of Geneva
email: menoud at cmu.unige.ch



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