E coli expression of eucaryotic protein

Patrick Grealish kallisti at merle.acns.nwu.edu
Mon Jul 17 20:17:58 EST 1995


HEllo, I am current starting another experiment where i'll need to produce
eucarotic protein in E. coli. i have used in the past the Ni-agarose
column method (ie quiagen) but i've found that even with careful column
washiung i was still getting some cotamination (kind of alot actually)
some i was hoping to utilize a different method of tagging (not the 6Xhis
tag). If anyone can help out i would greatly appreciate the assistance.
TIA

-- 
hail eris all hail discordia kallisti
Patrick Grealish
kallisti at merle.acns.nwu.edu



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