Plasmid loss after restreaking

R. P. Grant +44 1 865 221018 rpgrant at molbiol.ox.ac.uk
Tue Jul 18 11:57:12 EST 1995


(dinakar bhattramakki) writes:
> Hi netters,
> 
> I have a problem which I think was discussed in detail very recently. 
> Could any one post the conclusion of that discussion?
> "PROVERB IS NOT A PROVERB UNLESS YOU EXPERIENCE IT"
> 
> My problem: I transformed Sure18 cells with recombinant pBluescript 
> plasmid. Selected the white transformants on LB+Amp+X-gal+IPTG plates.
> I did a master plate of half a dozen transformants. I was unable to 

How do you know at this stage that you have transformants.  White colonies 
suggest you have interrupted the lacZ with your construct, but believe me, it 
ain't necessarily so.

Richard "The miniprep king"



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