Does RNA - PCR ??

Tracy Aquilla aquilla at salus.med.uvm.edu
Tue Jul 18 09:56:38 EST 1995


In Article <3ue8ub$cam at saba.info.ucla.edu>, walter
<wlech at medicine.medsch.ucla.edu> wrote:
>My understanding is that the RNA would be degraded rather rapidly under 
>the usual PCR conditions via magnesium-catalyzed hydrolysis.

Actually, RNA amplifies quite nicely with Taq polymerase. I have cloned
several cDNAs from total RNA this way. It works.
    tracy



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