Does RNA - PCR ??

Tracy Aquilla aquilla at
Tue Jul 18 09:56:38 EST 1995

In Article <3ue8ub$cam at>, walter
<wlech at> wrote:
>My understanding is that the RNA would be degraded rather rapidly under 
>the usual PCR conditions via magnesium-catalyzed hydrolysis.

Actually, RNA amplifies quite nicely with Taq polymerase. I have cloned
several cDNAs from total RNA this way. It works.

More information about the Methods mailing list