PCR from blood

Dr David E Barton dbarton at acer.gen.tcd.ie
Tue Jul 18 03:52:33 EST 1995


In article <3udb6l$7ms at ipgate.le.ac.uk>, neill gingles  <nag3 at le.ac.uk> wrote:
>Has anyone any methods or information on screening samples of blood for 
>a gene using PCR......

 We find that the simplest way is to make blood spots on 3MM paper, and put a 
 snippet (~1mm^2) directly into the PCR reaction. Start with 5min at 95C and
 off you go!

  | David Barton
  | National Centre for Medical Genetics
  | Our Lady's Hospital for Sick Children
  | Crumlin, Dublin 12, Ireland.
  | Tel 455 0515 Fax 455 8873




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