fungi in the incubator

[Andrey Mikhailow]

In article <3u7ks5$9uu at news.rrz.uni-koeln.de>, a2245094 at rrz.Uni-Koeln.DE 
says...
>
>
>Hello everybody,
>
>has anybody an idea how to prevent fungi as aspergillus and so on to 
>infect your cell cultures in the incubator ?
>I have to throw away cell cultures VERY often because of different sorts 
>of them.

I  prefer  to use a "Rocal" - oxydant type sterilizer for surgery in
water on the incubator flor; Potassiun bichromate in a  jacket water
and hygh concentration _fresh_ Amphotericine B in the culture
media. Hybridomas grows well even in serum-free conditions.

Andrey.