[Q] How to purify small PCR frg. ?

Peter Rudolph p_rudolph at MORPH.SPACENET.DE
Wed Jul 19 02:50:20 EST 1995


Hi netters,

recently a coworker of mine ran into a problem:
She created a library with PCR using randomized primers,
then she digested the fragment and want to ligate it into a vector.
The complete PCR fragment is 96bp, after digestion she gets
two fragments: 29 and 67bp. She separated them by gel
electrophoresis on a 2.5% AG.
The problem is now: How to purify the bigger fragment from the
gel slice with a really *high* yield?
She tried freeze-and-squeeze and Qiagen-gel-ex but got
only very low yields.
Any suggestions?

Any help will be highly appreciated!

TIA,

Peter.







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