DNA extraction from fungal mycelia
fosters at bbsrc.ac.uk
Wed Jul 19 06:02:38 EST 1995
In article <3uih7m$bq8 at oravannahka.Helsinki.FI>, you at somehost.somedomain (Yeshitila Degefu) says:
>I would appreciate if someone indicate to me a reliable protocol for
>extraction of DNA from fungal mycelium. I mean a protocol which yields
>clean DNA which digests completely by restriction enzymes. Thank you in
>advance for your help
Try using the method of Lee and Taylor (see full reference below),
I've found this works perfectly well, producing a good yield of clean
DNA perfectly suitable for restriction digestion and PCR work.
If you have trouble finding this reference then mail me directly
with your E-Mail address and I'll mail you back with the (modified)
protocol that we use.
Lee, S.B. and Taylor, J.W., (1990) Isolation of DNA from fungal
mycelia and single spores. In: PCR Protocols. A Guide to Methods
and Applications (Ed. by M.A. Innis, D.H. Gelfand, J.J. Sninsky
and T.J. White), pp 282-287. Academic Press, San Diego.
Cheers, Sim. (fosters at bbsrc.ac.uk)
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