exposing tritium gels

Wolfgang Schechinger u7k0201 at sunmail.lrz-muenchen.de
Wed Jul 19 12:55:50 EST 1995


Stephen_Lasky at brown.edu (Stephen R. Lasky, Ph.D.) wrote:
>In article <f.a.cassidy-1807950943510001 at bcs93.bham.ac.uk>,
>f.a.cassidy at uk.ac.birmingham (Fiona Cassidy) wrote:
>
>> I am running SDS PAGE and IEF gels of proteins which I have labelled with
>> tritium, and would like to expose wet gels. I am wondering if wrapping
>> these gels in cling film/saran wrap/ cellophane or something similar will
>> cause any problems with the signal detection?  I'd be grateful to hear if
>> anyone has done this in the past
>> thanks
>
>I believe that tritium labeled proteins don't have enough energy to expose
>film in a reasonable amount of time (maybe ever) even if the film and gel
>are in intimate contact, therefore I have to assume that you are using an
>enhancing system such as ppo/popop (original fluorography recipe) or
>something like Enhance to boost the signal.  If this is the case, I think
>that the energy that is emitted has a wavelength between 370 and 530 nm
>(or somewhere close to that, I don't remember exactly) which is just
>light.  Therefore, I think that if you can see through the saran wrap, the
>light should pass through it also. 
>
>SRLasky

Take a look at the spectral sensitivity of your film. If you are yousing an ordinary xray film 
it maybe is not sensitive enough in the emission range of your dye.

Wolfgang




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