Artefacts on SDS PA gels

Ian Ross I.Ross at mailbox.uq.oz.au
Wed Jul 19 19:52:39 EST 1995


HI - I didn't see the original query but I can verify that these bands 
are almost always due to keratin - usually from human fingers but 
sometimes from the tissue source.  If from humans, the only solution is 
scrupulous cleanliness.  Usually this becomes a problem with silver 
stained gels which of course are so much more sensitive than coomassie 
gels.

Regards

Ian Ross
Centre for Molecular and Cellular Biology
University of Queensland
Australia 4072





More information about the Methods mailing list