Protein elution from Ab columns

Ian Ross I.Ross at
Wed Jul 19 19:39:55 EST 1995

Re band widening on SDSPAGE

My guess is salt concentration and/or pH variation  (but probably salt) 
due to the lyophilisation step.  The best thing to do if it really is a 
problem is to remove the salt via an acetone precipitation step before 
adding SDSPAGE loading buffer (resuspend in small amount of buffer, add 
4 volumes ice cold acetone, on ice for 5 min, spin hard, dry).

Ian Ross
University of Qld

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