Reusing "disposable" electroporation cuvettes
I.Ross at mailbox.uq.oz.au
Wed Jul 19 19:18:30 EST 1995
Yeah, we use BioRad cuvettes and they are also reusable many times AS LONG
AS YOU TAKE CARE OF THEM. We don't wash with any detergent, we just pop
them into 70% ethanol immediately after use, then a bit later we wash them
out with a jet of deionised water. We then store them immediately in 70%
ethanol in a container with a closed lid (so no evaporation of ethanol).
They can be used many times - we chuck them when they get all crackly on the
outside and the electrodes are getting burnt. If you don't watch them, and
get bacterial contamination it's the end of the road, especially for us
since we work on macrophages and LPS is a major problem. Good luck.
Centre for Molecular and Cellular Biology
University of Queensland
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