RNA gels - staining and denaturing

Ian Ross I.Ross at mailbox.uq.oz.au
Wed Jul 19 19:30:38 EST 1995


Re: EtBr staining of formamide gels

Formamide reacts with EtBr so you cannot stain the gels directly.  You 
need to wash out the formamide first (eg 30 min with 0.1M ammonium 
acetate, preferably 2 washes).  Then it should stain ok, although the 
intensity is always less than for DNA because of RNA being single 
stranded.

Ian Ross





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