DMSO in CaCl2 Competent Cells
klenchin at macc.wisc.edu
Wed Jul 19 18:51:42 EST 1995
<gddavis-1907951835120001 at ppp206.modems.uoknor.edu>,
gddavis at essex.ecn.uoknor.edu (Greg Davis) wrote:
>Can anyone tell me what the function of DMSO is in the
>frozen competent CaCl2 competent cells?
My understanding is that it's not for preservation but for
the competence. As nobody knows what how E.coli
uptakes DNA, I guess it's a mistery what DMSO does here.
(For that matter: what CaCl2 does and how???)
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