Does RNA - PCR ??
Duncan Clark
Duncan at genesys.demon.co.uk
Wed Jul 19 06:25:02 EST 1995
In article: <DBxnyo.Gn3 at midway.uchicago.edu> rmoldwin at midway.uchicago.edu writes:
> I also have seen several reports of RT activity in Taq. Perhaps, it varies
> according to the manufacturer, and the specific origin of the Taq, e.g.
> recombinant/modified vs. wild type. In my lab, we very rarely see this RT
> activity when using standard Perkin Elmer recombinant AmpliTaq, which PE
> reports in their catalog to have "minimal, low" RT activity. AmpliTaq is
> modified from the wild-type Taq, but I can't determine what the difference is
> from their literature.
AmpliTaq is not modified at the aa level just a recombinant version with
modified codon usage. It has the same level of RT activity ie low.
As far as I am aware all thermus polymerases show a level of RT activity, but
some are better than others ie Tth from Thermus thermophilus HB8. Most have
an extremely poor level of RT activity.
On a similar note all thermus pols have the terminal transferase activity ie
they add a single base to the 5' end of the PCR product which is usually (but
not always) a T.
Duncan
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