Help: Blotting DNA from acrylamide gel

Frances Hannan Zoology flh at
Wed Jul 19 08:52:09 EST 1995


>> This is a real stumper for me.  I need to make a Southern blot of some
>> PCR products after separating them by size in a polyacrylamide gel.  

Did you denature the DNA prior to transfer ala Southern blots from agarose?
May be worth a try...

Frances Hannan                                       
BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
Phone (0223)336663, FAX (0223)461954                 
flh at                               

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