Help: Blotting DNA from acrylamide gel

Frances Hannan Zoology flh at mole.bio.cam.ac.uk
Wed Jul 19 08:52:09 EST 1995


>SBROWN at GAES.GRIFFIN.PEACHNET.EDU (Stuart Brown) wrote:

>> This is a real stumper for me.  I need to make a Southern blot of some
>> PCR products after separating them by size in a polyacrylamide gel.  

Did you denature the DNA prior to transfer ala Southern blots from agarose?
May be worth a try...


-- 
Frances Hannan                                       
BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
Phone (0223)336663, FAX (0223)461954                 
flh at mole.bio.cam.ac.uk                               



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