Artefacts on SDS PA gels

Mark Lamkin mlamkin at acs.bu.edu
Wed Jul 19 08:25:18 EST 1995


A while back, some one asked for help in explaining the presence of 
bands on SDS polyacrylamide gels with app. mol. wts. of between 50-60 
KD. As I recall, at least two opinions were provided in responses: 1) 
contamination due to keratin and 2) a contaminant in 
b-mercaptoethanol.

I have a student who is experiencing the same problem and we have 
traced it to either of the above. Can anyone provide references for 
these annoying bands.



Dr. Mark Lamkin
Boston Unversity School of Graduate Dentistry

"Saliva: A little bit goes a long way!"



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